Background: Aging is a multifactorial phenomenon that is characterized by a decrease in physiological functions that increases with age. Free radicals cause oxidative stress, which plays a role in the aging process. The accumulation of reactive oxygen compounds increases matrix metalloproteinase-2. Collagen can be directly destroyed by reactive oxygen compounds or through the induction of MPPs. Tomato fruit is an antioxidant, which acts as a binder and damper of free radicals that activate collagen damage. This study aims to assess the effect of tomato juice on the expression of MMP-2 and type-1 collagen on the vaginal wall of the menopausal rat.

Methods: This was a true experimental study, using Sprague-Dawley rats and tomato juice (Solanum lycopersicum). Expression of MMP-2 and type-1 collagen was examined using immunohistochemistry staining. Twenty-four healthy female rats, aged 4 months weighing 150-200 grams, were divided into 4 groups, each group as many as 6 rats. The negative control group (NC) was a group of rats with a sham procedure, given aquades for 28 days. The positive control group (PC) was bilaterally ovariectomized rats, given aquades for 28 days. The first treatment group (P1) was bilaterally ovariectomized rats, given tomato juice with a dose of 11 g / 200 g BW / day. The second treatment group (P2) was bilaterally ovariectomized rats, given tomato juice with a dose of 15 g / 200 g BW / day. Data analysis used the One-way ANOVA statistical test (α = 0.05) and different tests between groups using Games-Howell and Tukey HSD. The association between tomato juice and the expression of MMP-2 and type-1 collagen used regression tests.

Results: This study found that the lowest expression of MMP-2 was NC group (2.07 ± 0.84), and the highest was the PC group (5.72 ± 1.91). The mean MMP-2 expression in the treatment groups was lower than the PC group but still higher than the NC group. There was a significant difference in MMP-2 expression between groups (p = 0,000). There was a negative correlation between tomato juice administration and MMP-2 expression (b = -0.592). The lowest type-1 collagen expression was PC group (3.83 ± 1.44), and the highest was the P2 group (7.27 ± 2.21). The mean type-1 collagen expression in the treatment groups was higher than the PC group. There were significant differences of type-1 collagen expression between groups (p = 0.012). There was a positive correlation between tomato juice administration and type-1 collagen expression (b = 0.682).

Conclusions: Tomato juice administration can reduce the expression of MMP-2 and increase the expression of type-1 collagen on the vaginal wall of the menopausal rat. The higher the dose of tomato juice, the higher the expression of type-1 collagen.

Background: The presence of IgM, IgG and IgE antibodies has been widely used as important markers for the differentiation of acute and chronic infection in many infectious diseases, but limited studies reported on Cysticercus bovis infection are available. This study aimed to determine the IgM, IgG and IgE responses of mice against crude cystic fluid, p14, p31 and p71 antigens as the possible diagnosis of acute and chronic cysticercosis in hosts.

Methods: Six mice were immunized four times at 10 days interval with Cysticercus bovis. Individual p14, p31 and p71 proteins were separated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and elution of proteins from gel with elution buffer. The crude cystic fluid, p14, p31 and p71 were used as antigens for enzyme-linked immunosorbent assay (ELISA) to measure the IgM, IgG and IgE antibody titers in mice sera before and after the first, the second, the third and the fourth immunizations.

Results: No IgM, IgG and IgE against crude cystic fluid, p14, p31, and p71 was detected in sera samples collected before the first immunization. Moderate to high titers of IgM antibodies against crude cystic fluid, p14, p31 and p71 were identified following the first, the second, the third and the fourth immunizations. IgG titers against crude cystic fluid, p14, p31 and p71 was still at a low level following the first immunization and started to increase following the second, the third and the fourth immunization. Generally, low titers of IgE antibodies against crude cystic fluid, p14, p31 and p71 were detected after each immunization. IgE responses only increased slightly against crude cystic fluid and p71 antigens after the third and the fourth immunizations. The IgM, IgG and IgE responses of mice against crude cystic fluid and p71 antigens were higher than those against p31 and p14 antigens.

Background: Breast cancer is one of the most common malignancies. The relationship between the pathogenesis of breast tumours and their invasive behaviour is an important issue that has been discussed recently. ALDH1 and TGFb2 genes can be mentioned among the most crucial signalling and molecular pathways that play an active role in tumour invasive behaviour. The aim of this study was to determine the expression level of ALDH1 and TGFb2 genes in breast cancer and its relationship with histology in breast cancer patients.

Method and material: This cross-sectional study was done on 65 breast cancer patients who had been referred to the Army 501 hospital during the years of 1992-1994. Data was collected using the patient records, and the results of immunohistochemically staining were obtained in the laboratory. Data were analyzed by SPSS software using t-test, Chi-square and Fisher tests. The level of significance was considered to be 0.05.

Results: In this study, we assessed the expression of these genes in tissue samples of 65 patients with breast cancer and its relation with the histology and clinical progress. The expression of ALDH1 in the malignant breast tissue was 83.5% (55 out of 65 samples) and the expression of TGFb2 gene showed 54 samples (83.1%) were positive. There was a significant relationship between lymphatic drainage and ALDH1 expression.

Background: Remains not fully clear the impact of intestinal chelators on microrheological violation of red blood cells during the early stages of obesity. It dictates the necessity of experimental investigations' fulfilment on laboratory animals with just developed obesity in them.

Method: Ninety-four of healthy male-rats of Vistar line at the age of 2.5-3 months took into investigation. Twenty-nine animals of them had experienced no impacts and composed the control group. In 65 rats after their putting into small cages (one specimen - in a cage) during 30 days there was developed OB as the result of giving them of high-caloric diet from combined feed (47%), sweet condensed milk (44%), vegetable oil (8%) and vegetable starch (1%). Of the 35 rats received a day for 60 days of activated carbon 25 mg/kg into a stomach. There used biochemical, haematological and statistical methods of investigation.

Results: At obesity development in rats, there found a steady decrease of erythrocytes-discocytes quantity in blood. It accompanied by increase of reversibly and irreversibly changed erythrocytes' quantity in examined animals' blood. At obesity development in rats there was found quick rise of erythrocytes' sum in aggregate and these aggregates' quantity at lowering of free erythrocytes' number. During experimental obesity modelling we noticed very early in rats' blood decrease of erythrocytes-discocytes' quantity, level rise of their reversibly and irreversibly varieties with strengthening of their aggregative ability. It takes place in the background of weakening of erythrocytes' antioxidant protection and activation of lipids' peroxidation in them.

Conclusion: Application of activated carbons capable of rats with experimental obesity resolve arisen they breach microrheological properties of erythrocytes. It is clear that in obesity treatment regimens must be intestinal chelators, capable of positively affect many broken with this pathology haematological indices.

Background: PCOS typically characterized by a decreased level of GDF9 and BMP15, which contribute to follicular arrest. Recent research related to stem cells showed that conditioned media-mesenchymal stem cell (CM-MSC) might have a positive role in folliculogenesis. Therefore, this study aimed to assess the effectiveness of CM-MSC towards oocyte maturity

Method: A quasi-experimental design was used to examine the outcome of 3 different interventions, namely group A (4-hour incubation in standard media), group B (24-hour incubation in standard media), and group C (24 hours incubation in conventional media with addition of CM-MSC). Nuclear and cytoplasmic maturity, along with GDF9 and BMP15 levels were measured and analyzed.

Results: Sixty-three patients at an infertility clinic, RSUP Dr. Sardjito, Yogyakarta was recruited and a total of 292 germinal vesicles were obtained to start in vitro maturation procedure. Multivariate analysis showed group C has OR 6.9 (3,3–14,41) to obtain metaphase II oocyte than group B (p <0,0001). Infertility causes, insulin resistance, and maternal age risk are other factors that significantly influence oocyte maturity outcome (p <0.001; p = 0.005; p = 0.017). For the oocyte morphology outcome, no significant effect was obtained from the intervention (p> 0.05). Group C has higher GDF9 levels (Δ mean = 3.31) and BMP15 (Δ mean = 1.52) compared with group B (p <0.001; p = 0.006).

Conclusion: It can be concluded that 24-hour incubation in CM-MSC was effective to induce oocyte maturation. However, other factors, such as infertility causes, insulin resistance, and maternal age, should also be considered.

Introduction: Urinary tract infection (UTI) is the second-largest of the world’s health burden just behind the respiratory tract infection. Pregnant women are at risk of suffering from urinary tract infection due to anatomical and physiological changes along the urinary tract. The study aims to find out the influence of maternal age and gestational age to UTI in Primary Health Care Center Lempake.

Method: The research method was cross sectional with total sampling and using secondary data from the register book of emergency unit in Primary Health Care Center Lempake including all UTI cases in pregnancy from all pregnant patients undergone urinalysis from January 2017 until October 2018 (48 patients). It was then analyzed using logistic regression with STATA version 14.

Result: Most of subjects distribution were maternal age group 26-35 years old (39.6%), third trimester of pregnancy (66.7%), and negative result of bacteriuria (81.3%). Maternal age group 26-35 years old was the the most one suffering from UTI/positive result of bacteriuria (55.6%) and had tendency 5.4 times to suffer from urinary tract infection (p=0.147; CI=0.56-51.71; OR=5.36) while gestational age in third trimester showed significant influence to urinary tract infection (p=0.05; CI=0.03-1.00; OR=0.18). The prevalence of urinary tract infection in this research was 18.7%. 

Conclusion: There were significant influences among maternal age and gestational age to UTI in Primary Health Care Center Lempake.

Background: Prostate and colorectal cancer are the second and third most common cancer in male. Radiotherapy is performed as treatment option for both cancers. Thus, lead to the increasing case of radiotherapy-induced urethral stricture. Recurrent stricture post-correction commonly occurs.

Methods: Six relevant English literatures were found throughout online database published between 2011-2017. PICO is used to identify components of clinical evidence to create systematic review. Subjects include 222 radiotherapy-induced urethral stricture patients due to prostate and colorectal cancer who undergone various treatment modality options.

Results: Radiotherapy modality performed in sample population were External Beam Radiotherapy (EBRT) (44.1%), Brachytherapy (BT) (31.5%), EBRT/BT (16.2%), Adjuvant EBRT (6.8%), salvage EBRT (0.9%), and proton beam (0.5%). Strictures were found in bulbomembranous-urethra (64.4%), bulbar-urethra (17.1%), posterior-urethra (10.8%), membranous-urethra (5.4%), vesico-urethra (1.4%), and pan-urethra (0.9%). Known mean onset for urethral-induced radiotherapy is 5.6 years. Treatment options include anastomosis urethroplasty (61.3%), Buccal Mucosa Graft Urethroplasty (23.4%), urolume stent (10.8%), penile island flap onlay (2.2%), Genital fasciocutaneous skin flap (1.4%), and perineal flap urethroplasty (0.9%). Known mean onset for urethral stricture recurrence is 10.8 months.

Conclusion: Urethral strictures commonly occur after radiation therapy for prostate and colorectal cancer. Urethroplasty is the preferable treatment option. The recurrence onset for urethral stricture post urethroplasty is shorter than urethral strictures in general.

Background: Renal function recovery in postrenal acute kidney injury (AKI) patient is not always achieved and risk factor for chronic disease. Factors that affect of kidney function recovery include duration of symptoms, AKI stage and degree of hydronephrosis. The aim of the study is to determine the correlation of predisposing factors of renal function recovery in postrenal AKI patient.

Methods: A prospective observational analytic study with a cross-sectional study approach that involves postrenal AKI with urinary tract stones formers from August to November 2017. Renal function recovery was assessed by comparing creatinine levels before obstruction and after obstruction (3rd and 7th day) and be correlated by the duration of symptoms, AKI stage, and degree of hydronephrosis.

Results: 66 patients were included in the analysis of this study. Renal function recovery was found in 29 patients (43.9%) at 3rd day and 47 patients (71.2%) on the 7^th-day post obstruction. There was a significant difference in mean creatinine levels before and after obstruction (p <0.0001). Both duration of symptoms, AKI stage, and hydronephrosis showed correlation on renal function recovery on 3rd and 7th day after obstruction (p < 0.05). In logistic regression analysis, hydronephrosis was the most correlated predisposing factor for renal function recovery in 3rd and 7th day after obstruction (p = 0.0001).

Conclusion: Factors duration of symptoms, AKI stage, and degree of hydronephrosis significant correlated to renal function recovery after post-renal AKI. Degree of hydronephrosis was the most correlated predisposing factors.

Background: Chronic inflammatory process that may cause acute myocardial infarction (AMI) has been known to play an important role in the pathogenesis of atherosclerosis and atheroma plaque instability. Based on inflammation process, several previous studies showed contradictory results in finding a relationship between infection H. pylori with the formation of atherosclerosis through the establishment of immunoglobulin G (IgG) anti-H. pylori-HSP60II3 (IgG anti-Hp-HSP60II3), and neopterin level due to oxidative stress. This study aims to know whether IgG anti-Hp-HSP60II3, IFN-γ and neopterin are risk factors of cardiovascular events (CVE) in patients with ACS.

Methods: A prospective cohort study was conducted among 66 patients to determine the levels of IgG anti-Hp-HSP60II3, IFN-γ, and neopterin in patients with ACS as well as the levels of IgG anti-Hp-HSP60II3, IFN-γ, and neopterin. ACS patients were grouped into two groups: patients with ACS with positive prognostic factors (high levels of IgG anti-Hp-HSP60II3, IFN-γ, neopterin, and hs-CRP) and the group of patients with ACS without prognostic factor (low level of anti-IgG levels hp-HSP60II3, IFN-γ, neopterin, hs-CRP), and then were observed for 6 months while in the hospital. Data were analyzed using SPSS version 20 for Windows.

Results: In observations over a period of 6 months, found as many as 15 (22.7%) patients with CVE, which consisted of 11 (16.7%) patients’ vascular death, 3 (4.5%) patients had IMA and 1 (1.5%) patients experienced recurrent cardiac ischemia from 66 patients with ACS. Mean difference between the time of occurrence CVE subjects with high and low levels of neopterin are statistically significant (P<0.05). When confounding factors such as dyslipidemia, diabetes, hypertension, obesity, smoking, and age are controlled, only neopterin levels affect the incidence CVE with the incidence rate ratio of 6.46 (95% CI: 1.45-28.74; p = 0.014) and path analysis found the effect of neopterin on CVE incidence about 49,1%, p < 0.05. Kaplan-Meier analysis gives result that the average time of CVE on a group of subjects with high levels of neopterin is shorter (129.09 days) than the group of subjects with low levels of neopterin (168.33 days) (P=0.008).

Conclusion: This study found that the high level of anti-helicobacter pylori-heat shock protein 60II3, interferon-γ, and neopterin have a potential role in predicting risk factors of cardiovascular events in acute coronary syndrome patients

Background: Pneumococci cause mild or severe infections that begin with colonization in the nasopharyngeal area. Intranasal transmission is a natural route of bacterial infection in the host. This study aims to determine the type of serotype that can infect and provide an overview of inflammation in the lungs of mice after exposure to 107 bacteria S. pneumoniae serotypes 2, 3, 4, 19F and ATCC 6030 intranasally in animals try Balb / c mice.

Methods: True experimental study was conducted using Randomized Posttest Only Control Group Design among 30 Balb/c mice divided into 3 groups. The intervention used in this study was carried out twice, namely at 24 hours and 48 hours with 50 μl suspension of Streptococcus pneumoniae bacterial inoculum via intranasal drop by drop. Lung histopathology and CFU analysis of infected mice were evaluated. TNF-α was examined using ELISA. Data were analyzed using SPSS software version 17 for windows.

Results: The results showed that S. pneumoniae serotype 3 could infect Balb / c mice and found about 5x104 CFU (SD ± 7x104 CFU) at 101 dilutions and was still detected at 104 dilutions i.e. 0.5 CFU (SD ± 0.7 CFU) at 24 hours post-infection as well at 48 hours post-infection, accompanied by infiltration of neutrophil cells in the lung tissue at the same time. The TNF-α levels did not significantly differ between the treatment group (P>0.05)

Conclusion: The results of this study indicate that not all S. pneumoniae serotypes can infect experimental animals.

Lung cancer is known as the most common malignancy in the world in terms of incidence and death rate. GLOBOCAN data in 2018 showed that its prevalence reaches 11.6% with mortality reaching 1.7 million annually. The prevalence of lung cancer in Indonesia is also considerably high; there were 25,332 cases in men and 9,374 cases in women with mortality reaching 308,660 people. In general lung cancer is classified as non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). Although it is less common, SCLC has a worse prognosis with a 5 year survival rate of 6.4%. In addition, SCLC is also often diagnosed when it has metastasized or already reaced extensive stage (ES-SCLC). Although SCLC is classified as chemo-responsive cancer, the overall outcome of first-line and second-line therapy is still unsatisfactory with an ORR of 10-25%. One of characteristic of SCLC is genomic instability which relates to high level of mutations especially EGFR mutation that strongly correlate with therapeutic outcome. Therefore, targeting EGFR mutation is a sensible and potential field in developing SCLC therapy. EGFR-CpG-ODN nanovaccine is one of the potential therapeutic choices that exploit this mutation. EGFR CpG ODN nanovaccine could inhibit resistance to EGFR TKI, increased dendritic cell maturation, inhibit cancer cell proliferation and apoptosis, as well as enhance the anticancer immune response. Therefore, this therapeutic approach is a promising future therapy for SCLC petients that could improve patient’s survivability.

Introduction: Women readiness and self-efficacy for pregnancy and childbirth is an important issue which usually assessed by using CBSEI-32 but currently no evaluation is ever conducted nor Indonesian version of this instrument ever developed to enhance subject understanding. Therefore, this study aimed to develop the Indonesian Language version of CBSEI (childbirth self-efficacy inventory) instrument.

Method: A quantitative study was conducted by involving 73 pregnant mothers in their trimester III either as the primigravida or as multigravida. The selected respondents are those who lived in the working area or visited several health care centres in the area between July-August 2018. The content validity was tested by using Aiken V analysis and the construct validity was assessed using Kaiser-Meyer-Olkin (KMO) and Bartlett’s test. Finally, the reliability test was conducted using Alpha Cronbach.

Result:  Analysis revealed that the Aiken V value of the instrument was in the range of 0.67-1 for the outcome expectancy and 0.75-1 for efficacy expectancy. 16 items in outcome expectancy showed KMO value > 0.5 with MSA value between 0.648-0.957.  Meanwhile, 16 questions in efficacy expectancy also had KMO value > 0.5 with a MSA range between 0.798-0.937 respectively. The average values of the instrument validity for the outcome expectancy and efficacy expectancy were 0.740 and 0.726 respectively. Finally, Alpha Cronbach value for both outcome expectancy and efficacy expectancy was 0.94.

Conclusion: According to the analysis, it can be concluded that CBSEI-C32 in Indonesia language version was valid and reliable instrument to be applied in pregnant mother in Indonesia.

Background: Public health cadres can play an important role in improving disaster response and recovery. However, a multidimensional instrument is needed to assess the levels of disaster knowledge for community health cadres. This instrument is important to develop a complete and effective educational program for disaster preparation for health cadres. Therefore, this study aimed to test validity and reliability of the disaster preparedness knowledge instrument for health cadres in areas prone to volcanic disasters.

Methods: A cross-sectional study was conducted by involving 300 respondents. The instrument was developed by conducting literature review followed by completing a qualitative study. We reduced the original instrument from 50 items to a final instrument comprising 41 items, classified into 6 categories: 1) early warning [16 items]; 2) disaster triage [4 items]; 3) basic life support [7 items]; 4) first aid [7 items]; 5) search and rescue [5 items]; and 6) logistic, communications and team organization [2 items].  Instrument validation was assessed by using Pearson Product moment and the reliability was estimated by using internal consistency reliability.

Results: 300 respondents were involved in this study with all respondents were women aged between 40-49 years old. All of them were either health cadre or high school students. In instrumental testing, all 41 items of the instrument were confirmed through content validity (Pearson Product Moment). Using internal consistency reliability, the study found reasonable inter-item reliability for all items (Cronbach’s α: 0.785-0.807).

Conclusion: Higher score on disaster triage aspect suggest that health cadres have pivotal role in emergency care during disaster. The finding revealed that disaster triage technique and skills for health cadres or lay man is very important.

Introduction: Leprosy is a chronic granulomatous infection caused by M. leprae with high affinity to Schwann cell. Primary concern of leprosy is disability due to progressive nerve damage so that patients and their families are sometimes faced with social stigma and discrimination. This can be caused by late detection and treatment of nerve damage. Electroneuromyography (ENMG) as the gold standard for nerve damage detection has limitations in terms of cost and operational procedures, so there is a need for simpler and cheaper method such as monofilament test. This study aims to determine the validity of monofilament test compared to ENMG for the detection of peripheral neuropathy in leprosy.

Method: Study design using analytic method with cross-sectional approach, a total 40 patients including paucibacillary and multibacillary type of leprosy who met inclusion and exclusion criteria were recruited by consecutive-sampling. All subjects undergo monofilament test at Dermato-Venereology Clinic of Sanglah General Hospital Denpasar and electroneuromyography test at Neurology Clinic of Sanglah General Hospital Denpasar from December 2018 to February 2019. The data was analyzed to determine sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV).

Result: The result of this study revealed that monofilament test had an overall sensitivity of 67.2% and specificity of 77.5%. High sensitivity and specificity were found in the sural nerve (85.7% and 75%) and radial nerve (82.7% and 80%)

Conclusion: monofilament test can be considered for early detection of peripheral neuropathy, especially for sural and radial nerve.

Background: Surgical intervention such as video-assisted thoracoscopic surgery (VATS) has shown the benefit of reducing morbidity and mortality in the management of pleural empyema. The objective of the study was to investigate preoperative factors that could predict the need for VATS rather than chest tube thoracostomy.

Methods: This is retrospective observational study of consecutive patients diagnosed with pleural empyema admitted to the Dr. Zainoel Abidin Hospital, Banda Aceh in period of 2015 to 2017. The demographic, clinical dan laboratory data of the patients were evaluated from hospital medical records.

Results: A total of 48 consecutive patients were identified. The mean age of the patients was 34.3 ± 20.4 years; and 31 (64.6%) were male. Twenty-six patients underwent VATS decortication and twenty-two patients underwent chest tube thoracostomy. Patient demographics were similar between VATS decortication and chest tube thoracostomy group. The significant preoperative factors associated with the VATS were serum albumin level and leukocytes (p <0.05). The mean serum albumin level was 2.81 ± 0.44 g/dL for the VATS decortication group vs 3.06 ± 0.41 g/dL for the chest tube thoracostomy group (p = 0.047). In addition, serum leukocyte level was higher in VATS decortication group compared to chest tube thoracostomy group (21,600 mm^-3 vs 12,900 mm^-3; p = 0.024).

Conclusion: We demonstrate that a low serum albumin level or an elevated leukocyte level may represent as preoperative factors in which surgical treatment may be required in the management of pleural empyema.

Introduction: Lung cancer which is a major cause of cancer deaths provides a perspective on the importance of the diagnosis of cancer since there is suspicion that influences the clinical diagnosis of lung cancer. Imaging modalities such as the use of contrast CT-Scan are very helpful in giving initial imaging before confirming cytopathological examination. The purpose of this study was to determine the value of the diagnostic tests possessed by CT-Scan compared to the gold standard cytopathological examination in patients with clinical diagnosis of lung cancer.

Methods: This study used a diagnostic study design with a retrospective approach at Zainoel Abidin General Hospital, Banda Aceh-Indonesia during the period June - August 2018. Analysis of diagnostic tests using cross-tabulation then calculation of sensitivity, specificity, positive predictive value (PPV), negative predictive calculations value (NPV), positive likelihood ratio (LR +), negative likelihood ratio (LR-), and accuracy were performed.

Results: Most CT-scan findings were pulmonary mass (59.3%) and the most cytopathological findings were squamous cell carcinoma (47.5%). The CT-Scan diagnostic test value compared with the gold standard cytopathological examination in the form of sensitivity, specificity, PPV, NPV, LR +, LR-, and accuracy was 75%, 30.4%, 64.4%, 50%, 0.054, 0.822 and 61% respectively.

Conclusion: Lung cancer could manifest as a wide spectrum of radiological presentations. CT-Scan provide a high sensitivity and accuracy of diagnostic in person with clinical presentation of lung cancer.

Background: Secondary hyperuricemia can increase the risk of renal dysfunction in the form of fibrosis and glomerulosclerosis mediated by TGF-β signalling. Non-pharmacological therapy through high dietary antioxidant intakes can help in controlling and preventing renal dysfunction by suppressing TGF-β expression. This study aimed to analyse the effect of red guava juice (Psidium Guava Linn.) on TGF-β protein expression in mice (Mus Musculus) kidneys with secondary hyperuricemia model.

Method: This was an experimental study with a post-test only design. Forty-eight mice (Mus Musculus) were choosen as samples, which were distributed into six different treatment groups. The mice were induced with potassium oxonate for 14 days and given red guava juice for 21 days. TGF-β protein expression in mice (Mus Musculus) kidneys was identified through immunohistochemistry using TGF-β antibodies. The preparation was observed in the Anatomical Pathology Laboratory, Faculty of Medicine, Universitas Sebelas Maret using the Olympus XC10 light microscope. Data were statistically analysed using the Kruskal Wallis test, followed by the Post-Hoc Mann Whitney test.

Results: The results of this study showed that the administration of red guava juice had a significant effect on TGF-β expression (p = 0.001). There was a significant difference between the negative control treatment and treatment of red guava juice at a dosage of 5 ml/kg/day (p = 0.043).

Conclusion: It can be concluded that the administration of red guava juice in mice (Mus Musculus) with secondary hyperuricemia model can suppress TGF-β expression, and therefore, can be used as non-pharmacological therapy.

Background: Chronic rhinosinusitis is a chronic inflammation of the nasal mucosa and paranasal sinuses that can occur due to multifactorial and affect the quality of life of the sufferer. IL-8 is a proinflammatory cytokine that can significantly increase in chronic rhinosinusitis. Standard therapy and nasal washing with saline isotonic can reduce symptoms that complained of the patient and as well as the effect on changes in interleukin-8 as a proinflammatory mediator. This study aimed to determine the effectiveness of nasal irrigation with NaCl 0.9% against IL-8 level and quality of life in chronic rhinosinusitis patients.

Methods: This study is an experimental study with a pre-post test control group design. The subject of the study was 30 people divided into two groups with simple randomisation, each group of 15 people in which the control group received standard therapy and treatment group received standard therapy and nasal irrigation with saline isotonic for two weeks. Quality of life assessment was conducted with SNOT-22 and interleukin-8 examination before and after therapy in each group.

Results: There was no significant difference in SNOT-22 (p=0.227) of both groups before and after therapy. There was a significant difference in the ear and facial symptoms (p=0.025) and psychological (p=0.043) of both groups before and after therapy.

Conclusion: Standard therapy and nasal washing with saline isotonic in patients with chronic rhinosinusitis give a better effect compared to standard therapy without nasal washing with saline isotonic in terms of inhibiting elevated interleukin-8 levels.

Background: Hepatocellular carcinoma (HCC) is the six most common cancer and the fourth leading cause of cancer-related death worldwide. Recent reports have suggested an increasing prevalence of HCC without evidence of hepatitis B and C infection (non-viral HCC).

Methods: This was a descriptive study with cross-sectional approach that aimed to describe the distribution of viral and non-viral risk causes of hepatocellular carcinoma cases at Arifin Achmad General Hospital (AAGH), Riau Province in the period of 2013-2017. Data were obtained from medical records of HCC patients using total sampling method.

Results: We included 129 cases of whom 64 (49,6%) were associated with viral causes and 65 (50,4%) were  non-viral HCC. Bivariate analyses showed that there was no age difference between viral and non-viral HCC patients but the prevalence of non-viral HCC was significantly higher in females than males (odds ratio [OR], 3,12 ; 95% confidence interval [CI], 1,2-8,1; p=0,016). In addition, patients with alpha-fetoprotein (AFP) <400 ng/mL were more frequently associated with non-viral HCC compared with those with elevated AFP ³400 ng/mL (OR, 3,71; 95%CI, 1,49-9,26; p=0,004).

Background: Tekelan leaves (Chromolaena odorata) has been commonly used traditionally as a medicine. It is known that Tekelan leaves have bioactivity as an antibacterial. These leaves have antibacterial activity for both gram-positive and negative bacteria so that it can be made for semi-solid preparation namely hand sanitiser gel (HSG). This study aims to determine the optimum concentration of HSG base with TLE and determine the physical characteristics of the optimum formula.

Methods: Sample extraction was done by maceration method with 96% of ethanol (1:10). Dechlorophyllation with n-hexane also used to separate the chlorophyll and to get a better colour of extract while it formulated in gel form. Optimisation of the gel is conducted with the simplex lattice design (SLD) method. SLD method is available in Design-Expert software. The method was designed to optimise the formula with different concentration of different component (Gliserin, TEA, and Carbopol) so it can produce the optimum formula. The evaluation of HSG involves spreadability test, sticky power and pH test. Furthermore, the acceptability evaluation was done by 20 respondents.

Results: The optimum gel base formula is consists of 1.860% glycerin, 2.901% TEA, and 0.739% carbopol with spreadability 3.673 cm, sticky power 1.181 s, and pH 7.187. The acceptability of the C. odorata HSG also compared with one of the commercial HSG. The percentage of acceptability of C. odorata HSG is 75.25% and 80.25% for the commercial HSG.

Conclusion: HSG preparations of TLE which are formulated using optimum base formula have good physical properties.

Background: Healthy, intelligent, and highly competitive children are the asset of a nation. Indonesia as one of the developing countries which level of fish consumption is still low, including children fish intake. Children fish consumption in Surakarta, Central Java Province has potential to improve. Growth and brain development persist in childhood and adolescence at 9-10 years. That age range is the beginning of the peak growth (growth spurt) that requires the intake of fat and protein to support the growth and development of the brain and fish is a potent food source of protein and omega-3 fatty acids.

Aim: This study aims to analyze the relationship between fish consumption frequency and fish type with children intelligence level at aged 9-10 years.

Method: The design of this study was cross-sectional involving 112 elementary school children grade 4 in Surakarta, Central Java, Indonesia. Data were collected using questionnaires, 1 x 24-hour food recall form, semi-quantitative FFQ (Frequency Food Questionnaire), anthropometry, and intelligence level using Culture Fair Intelligence Test (CFIT-2A) method. The results were analyzed by multivariate analysis with multiple logistic regression.

Results: Multiple logistic regression analysis on the intelligence level of children aged 9-10 years showed that the frequency of fish consumption has a value of p = 0.170 and fish species has a value of p = 0.259, while confounding factors in the form of mother education and income of parents have their respective p-value of 0.011 and 0.171.

Conclusion: There is no significant correlation between the frequency of fish consumption and type of fish to the intelligence level of children aged 9-10 years (p> 0,05). However, the probability of subjects whose frequency of consumption of fish >3 times/week to have intelligence in the intelligent category greater than 1.81 times the subject consuming fish ≤3 times/week, after controlling maternal education factors and income parents.

Background: C-reactive protein (CRP) is an acute phase protein that reacts as a non-specific systemic inflammatory marker. Recent studies indicate that high-sensitivity CRP (hs-CRP) is a strong risk factor for predicting ensuing cardiovascular events.

Objective: To investigate whether hs-CRP could serve as a parameter in predicting 6-month mortality and the likelihood of rehospitalization of patients with HF.

Method: A prospective cohort study observed a total of 44 patients admitted to RSUP Haji Adam Malik Medan diagnosed with primary heart failure (HF) from November 2016 to February 2017. Level of hs-CRP was analyzed at their initial assessment and subjects were followed up for 6 months for mortality and rehospitalization.

Result: Fourteen (31.8%) patients died and nineteen (42.3%) patients were rehospitalized during the 6 months follow up period. The mean value of hs-CRP was higher in the mortality group (4 group (4.386±1.75) than the survivor group (2.227±1.80), P=0.001. There was no significant difference in the mean value of hs-CRP between rehospitalized patients group (2.858±1.99) compared to the group that was not rehospitalized (2.759±2.15), P=0.880. The analysis using the receiving operating characteristic (ROC) curve obtained area under curve (AUC) of 81% (95% confidence interval [CI]: 0.68-0.94), P=0.001. Based on the ROC curve, an hs-CRP value of 4.25 result in a sensitivity value of 63% and a high specificity value of 90%, P=0.000.

Conclusion: Among HF patients, increasing level of hs-CRP with cut-off value of 4.25 can be used as a 6-month mortality predictor and have a sensitivity of 64% and specificity of 90%. There were no significant differences in hs-CRP level between patients with HF who experienced rehospitalization within 6 months and who did not, hence hs-CRP could not be used as a predictor of rehospitalization within 6 months in patients with HF.

Introduction: Leprosy is a chronic granulomatous infection with a variety of clinical spectrum caused by Mycobacterium leprae (M. leprae) that is highly influenced by host immune response. In leprosy, vitamin D acts as an immunomodulator through a VDR-mediated antimicrobial pathway that affects the innate immune system to bacterial killing. M. leprae inhibits VDR activity through the down-regulation of CYP27B1. Elevated 1.25(OH)₂D levels are required to modulate cathelicidin antimicrobial peptide (CAMP) production, which leads to reduced 25(OH)D level. This study aimed to analyze the difference in serum vitamin D level between leprosy patients and healthy people.

Methods: This research was an analytical observational study with a cross-sectional design involving 20 patients with new cases of leprosy and 20 controls. The diagnosis of leprosy was confirmed through physical examination and laboratory examination. We conducted blood sampling and measurement of serum vitamin D (25(OH)D) level in both groups using the chemiluminescence immunoassay (CLIA) method. The collected data were then processed and analyzed statistically using the Mann-Whitney test.

Result: In this study, we found that the mean serum vitamin D level in leprosy patients (22.27 ± 5.418 ng/mL) was lower than controls (33.00 ± 1.913 ng/mL), and the difference was statistically significant (p < 0.05). The mean serum vitamin D level in male leprosy patients (23.69 ± 4.034 ng/mL) was higher than females (16.55 ± 7.081 ng/mL), and was highest in patients aged 36 – 45 years (25.314 ± 2.2945 ng/mL).

Conclusion: Serum vitamin D level was significantly lower in leprosy patients than in controls. Serum vitamin D level in leprosy patients was higher in the male group and was highest in patients aged 36 – 45 years.