ORIGINAL ARTICLE

The alterations of XRCC1 gene’s polymorphism with a different SNP is involved in breast cancer

Varesh Shams Akhzari, Fatemeh Asgharpour–Dil, Mohsen Shoja, Fatemeh Dehbandi, Shahrbano Keyhaniyan, Roya Khaksar, Seyedeh Elham Norollahi, Abbas Ghayoumi Javinani, Sahar Ghazanfari, Ali Akbar Samadani

Varesh Shams Akhzari
Department of Biology, Islamic Azad University of Tonekabon Branch, Tonekabon, Iran.

Fatemeh Asgharpour–Dil
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.

Mohsen Shoja
Faculty of Para medicine, Semnan University of Medical Sciences, Semnan, Iran

Fatemeh Dehbandi
Gastrointestinal and liver diseases research center (GLDRC), Guilan University of Medical Sciences, Rasht, Iran.

Shahrbano Keyhaniyan
Department of Biology, Islamic Azad University of Tonekabon Branch, Tonekabon, Iran.

Roya Khaksar
Gastrointestinal and liver diseases research center (GLDRC), Guilan University of Medical Sciences, Rasht, Iran.

Seyedeh Elham Norollahi
Faculty of Medicine, Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran Faculty of Medicine, Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran

Abbas Ghayoumi Javinani
Dr Ghayoumi Medical Laboratory, Kashan, Iran.

Sahar Ghazanfari
Department of Mycology, Faculty of Medical Sciences,Tarbiat Modares University, Tehran,Iran.

Ali Akbar Samadani
Faculty of Medicine, Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran. Faculty of Medicine, Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran. Email: [email protected]
Online First: October 03, 2018 | Cite this Article
Akhzari, V., Asgharpour–Dil, F., Shoja, M., Dehbandi, F., Keyhaniyan, S., Khaksar, R., Norollahi, S., Javinani, A., Ghazanfari, S., Samadani, A. 2018. The alterations of XRCC1 gene’s polymorphism with a different SNP is involved in breast cancer. Bali Medical Journal 7(3). DOI:10.15562/bmj.v7i3.942


Background: One of the most serious and lethal type of cancer among women with a high mortality rate is breast cancer.                                                                                                    

Methods: Relatively, this study examined the alternations of polymorphism of XRCC1gene in codon of Arg 399 Gln on 150 patients who had breast cancer and 150 normal ones as control cases in ages between 25 to 35 and also 45 to 55 after obtaining patients consent alongside with confirmation of pathology and oncology department in Imam Sajad hospital, Ramsar. Correspondingly, the genetic analysis was accomplished by RFLP-PCR technique and also MSPI restricted enzyme was used in order to find the SNPs.

Results: The measured clinical qualifications of all patients were comprised: SD and Mean of age: 4.8 and 48, SD and Mean of menstrual age: 4.8 and 13, SD and mean of menopause: 4.8,49, SD and mean of height: 12 and 175, SD and mean of weight: 67.2 and 91, SD and mean of children’s numbers: 103 and 3.

 The final results have indicated that the polymorphism of XRCC1 gene in 150 patients which had breast cancer, had 76.67% heterozygosity (p= 0.03) and 27.87% homozygosity (p=0.02).                                                                                                                 

References

Ramalho M, Fontes F, Ruano Ls, Pereira S, Lunet N. Cognitive impairment in the first year after breast cancer diagnosis: A prospective cohort study. The Breast. 2017;32:173-8.

Ziv E, Tice JA, Sprague B, Vachon CM, Cummings SR, Kerlikowske K. Using Breast Cancer Risk Associated Polymorphisms to Identify Women for Breast Cancer Chemoprevention. PloS one. 2017;12(1):e0168601.

Tang X, Gao Y, Yu L, Lu Y, Zhou G, Cheng L, et al. Correlations between lncRNA-SOX2OT polymorphism and susceptibility to breast cancer in a Chinese population. Biomarkers in medicine. 2017;11(3):277-84.

Todorova VK, Makhoul I, Dhakal I, Wei J, Stone A, Carter W, et al. Polymorphic Variations Associated With Doxorubicin-Induced Cardiotoxicity in Breast Cancer Patients. Oncology research. 2017.

Al Zoubi MS, Zavaglia K, Mazanti C, Al Hamad M, Al Batayneh K, Aljabali AAA, et al. Polymorphisms and mutations in GSTP1, RAD51, XRCC1 and XRCC3 genes in breast cancer patients. The International journal of biological markers. 2017;32(3):e337-e43.

Grundy A, Richardson H, Schuetz JM, Burstyn I, Spinelli JJ, Brooks-Wilson A, et al. DNA repair variants and breast cancer risk. Environmental and molecular mutagenesis. 2016;57(4):269-81.

Mordukhovich I, Beyea J, Herring AH, Hatch M, Stellman SD, Teitelbaum SL, et al. Polymorphisms in DNA repair genes, traffic-related polycyclic aromatic hydrocarbon exposure and breast cancer incidence. International journal of cancer. 2016;139(2):310-21.

Sanjari Moghaddam A, Nazarzadeh M, Noroozi R, Darvish H, Mosavi Jarrahi A. XRCC1 and OGG1 Gene Polymorphisms and Breast Cancer: A Systematic Review of Literature. Iranian journal of cancer prevention. 2016;9(1):e3467.

Sanjari Moghaddam A, Nazarzadeh M, Sanjari Moghaddam H, Bidel Z, Karamatinia A, Darvish H, et al. XRCC1 Gene Polymorphisms and Breast Cancer Risk: A Systematic Review and Meta- Analysis Study. Asian Pacific journal of cancer prevention : APJCP. 2016;17(S3):323-30.

Norollahi S. The Role of MicroRNAs in Cancer Progression. J Clin Exp Oncol. 2016;5(2).

Norollahi SE, Hamidian MT, Shokravi M, Ajourlou M, Samadani AA. The Impression of Histone Modification and DNA Methylation in Gastric Cancer Development: Molecular Mechanism Approach. International Biological and Biomedical Journal. 2015;1(4):128-36.

Ding P, Yang Y, Cheng L, Zhang X, Cheng L, Li C, et al. The relationship between seven common polymorphisms from five DNA repair genes and the risk for breast cancer in northern Chinese women. PloS one. 2014;9(3):e92083.

Taheri N, Samadani AA, Vahedi L, Norollahi SE, Yazdani P, Ghasemi M, et al. Histopathological investigation of Skin Cancer and Study of CDKN2A Gene Polymorphism in Mazandaran Province, Iran. International Biological and Biomedical Journal. 2016;2(2):60-6.

Luo H, Li Z, Qing Y, Zhang S-H, Peng Y, Li Q, et al. Single nucleotide polymorphisms of DNA base-excision repair genes (APE1, OGG1 and XRCC1) associated with breast cancer risk in a Chinese population. Asian Pacific journal of cancer prevention : APJCP. 2014;15(3):1133-40.

Qian Y, Zhang J, Dong J, Wang F, Lin Y, Xu M, et al. Relationship between polymorphisms of X-ray repair cross-complementing group 1 gene Arg194Trp, Arg399Gln and susceptibility of breast cancer. Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]. 2010;44(3):242-6.

Krivokuca AM, Cavic MR, Malisic EJ, Rakobradovic JD, Kolarevic-Ivankovic D, Tomasevic ZI, et al. Polymorphisms in cancer susceptibility genes XRCC1, RAD51 and TP53 and the risk of breast cancer in Serbian women. The International journal of biological markers. 2015;31(3):e258-63.

Batar B, Guven G, Eroz S, Bese NS, Guven M. Decreased DNA repair gene XRCC1 expression is associated with radiotherapy-induced acute side effects in breast cancer patients. Gene. 2016;582(1):33-7.

Guo S, Mao X, Ming L. XRCC1 Arg399Gln polymorphism is not associated with breast cancer in Chinese. International journal of clinical and experimental medicine. 2015;8(7):10429-36.

Gong Y, Qi M, Chen J, Fang R, Mai C, Chen T, et al. XRCC1 Arg194Trp and Arg399Gln polymorphisms and risk of extrahepatic cholangiocarcinoma: a hospital-based case-control study in China. International journal of clinical and experimental medicine. 2015;8(10):19339.

Seibold P, Behrens S, Schmezer P, Helmbold I, Barnett G, Coles C, et al. XRCC1 polymorphism associated with late toxicity after radiation therapy in breast cancer patients. International Journal of Radiation Oncology* Biology* Physics. 2015;92(5):1084-92.

Sangrajrang S, Schmezer P, Burkholder I, Waas P, Boffetta P, Brennan P, et al. Polymorphisms in three base excision repair genes and breast cancer risk in Thai women. Breast cancer research and treatment. 2008;111(2):279-88.

Samadani AA, Nikbakhsh N, Fattahi S, Pourbagher R, Mir SMA, Kani NM, et al. RNA Extraction from Animal and Human's Cancerous Tissues: Does Tissue Matter? International journal of molecular and cellular medicine. 2015;4(1):54.

Norollahi SA, Kokhaee P, Rashidy-Pour A, Hojati V, Norollahi SE, Vahedi Larijani L, et al. Comparison of RNA extraction methods of breast and gastric cancer tissues. Crescent Journal of Medical and Biological Sciences. In Press;5(1).

Fattahi S, Langroudi MP, Samadani AA, Nikbakhsh N, Asouri M, Akhavan-Niaki H. Application of unique sequence index (USI) barcode to gene expression profiling in gastric adenocarcinoma. Journal of cell communication and signaling. 2017;11(1):97-104.


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