ORIGINAL ARTICLE

HEMATOPOIETIC PROGENITOR CELLS AS A PREDICTIVE OF CD34+ ENUMERATION PRIOR TO PERIPHERAL BLOOD STEM CELLS HARVESTING

Z. Zulkafli , R. Mustaffa, And S. M. Yusoff

Z. Zulkafli
Biomedicine program, School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Email: [email protected]

R. Mustaffa
Department of Hematology and Transfusion Medicine, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.

And S. M. Yusoff
Department of Hematology and Transfusion Medicine, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Online First: September 04, 2014 | Cite this Article
Zulkafli, Z., Mustaffa, R., Yusoff, A. 2014. HEMATOPOIETIC PROGENITOR CELLS AS A PREDICTIVE OF CD34+ ENUMERATION PRIOR TO PERIPHERAL BLOOD STEM CELLS HARVESTING. Bali Medical Journal 3(3): 112-115. DOI:10.15562/bmj.v3i3.85


Background: To date, the CD34+ cell enumeration has relied predominantly on flow cytometry technique. However, flow cytometry is time consuming and operator dependent. The application of the hematopoietic progenitor cells (HPCs) channel in Sysmex XE-2100, a fully automated hematology analyzer offers an alternative approach, which is with minimal sample manipulation and less operator dependent. This study evaluates the utility of HPC counts as a predictive of CD34+ enumeration prior to peripheral blood stem cells harvesting. Materials and methods: HPC, CD34+, white blood cell (WBC), reticulocytes (retic), immature platelet fraction (IPF) and immature reticulocyte fraction (IRF) were determined in 61 samples from 19 patients with hematological malignancies (15 lymphoma and 4 multiple myeloma patients) at Hospital Universiti Sains Malaysia (Hospital USM) who had received granulocyte-colony stimulating factor (G-CSF) and planned for autologous transplantation. Results: CD34+ count showed strong and significant correlation with HPC. The receiver operating characteristics (ROC) curve analysis revealed that HPC count > 21.5 x 106 / L can predicts a pre harvest CD34+ count of >20 x 106 / L with sensitivity of 77%, specificity of 64% and area under the curve (AUC) of 0.802. Conclusion: We concluded that HPC count can be a useful potential parameter in optimizing timing for CD34+ enumeration prior to leukapheresis.
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