The anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on against DMBA-induced mammary cancer in Sprague Dawley rat through the regulation of microRNA-21 expression
Ida Ayu Ika Wahyuniari
Department of Histology, Faculty of Medicine, Udayana University, Bali. Email: [email protected]
I G K Nyoman Arijana
Department of Histology, Faculty of Medicine, Udayana University, Bali
Ni Putu Sriwidyani
Department of Anatomical Pathology, Faculty of Medicine, Udayana University, Bali
Ida Ayu Dewi Wiryanthini
Department of Biochemistry, Faculty of Medicine, Udayana University, Bali,
Hery Suwito
Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya
Sitarina Widyarini
Department of Anatomical Pathology, Faculty of Veterinary Science, Universitas Gadjah Mada, Yogyakarta
Muhammad Ghufron
Department of Histology and Cell Biology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
Mustofa Mustofa
Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
Sofia Mubarika
Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
Background: The new anticancer is urgently needed due to the high resistance and recurrence of breast cancer. Previous study reported that a new chalcone derivative, (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on, has a potential cytotoxicity against T47D breast cancer cell line. In this study we investigated the anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on against DMBA-induced mammary cancer in Sprague Dawley rat and its effect on microRNA-21 expression.
Methods: Twenty four female rats were divided into 6 groups. The first group, G1 received corn oil. The groups 2 to 6 (G2, G3, T1, T2, and T3) were induced by DMBA (dissolved in corn oil) 20 mg/kgBW for 5 weeks. After breast nodule observed, G2 received vehicle and G3 received tamoxifen. Whereas, T1, T2, T3 received (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on with different doses, ie. 5, 15, and 45 mg/kgBW/day for 21 days, respectively. Tumor size was measured every weeks for 21 days and on day 22, plasma and breast tissues were collected to examine the miR-21 expression by qRT-PCR and histopathological feature, respectively.
Result: The result showed that significantly decreased tumor growth (p<0.05) and better histopathological malignant grading in G3, T1, T2, T3 were observed. Moreover, significantly reduced miR-21 relative expression (p<0.05) in G3, T1 and T2 were also observed.
Conclusion: (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on has potential anticancer activity on DMBA-induced mammary cancer in Sprague Dawley rat through its activity on miR-21 expression. Hence, (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on might be a new anticancer candidate in the future.
