Link of Video Abstract: https://youtu.be/TRnFAXBESHc
Background: Coronavirus disease 2019 (COVID-19) has become a significant disease causing a pandemic worldwide. Many variants were detected within the globe and may have an important role in transmission capability or reinfection conditions. The ‘unknown’ level of immunity’s protection from previous infection and the more virulent virus could lead to reinfection. Using the whole genome sequence, we can analyze the genomic insight within the first and second infections to help understand the reinfection pattern of COVID-19.
Methods: In this study, we investigated three COVID-19 cases with reinfection for primary and secondary infection each. The SARS-CoV-2 confirmation was conducted using qPCR with multiplex gene targets Orf1ab and E. Confirmation of reinfection was done by genomic analysis. Library preparation and sequencing were done using ARTIC protocol using nanopore technology.
Results: There was a wide variety of mutations between two episodes of infection and different clinical manifestations in each episode.
Conclusions: The result concluded that reinfection happened in worsened clinical symptoms and distinct genomic variation in each episode of infection