Chondrogenic Differentiation Capacity of Human Umbilical Cord Mesenchymal Stem Cells with Platelet Rich Fibrin Scaffold in Cartilage Regeneration (In Vitro Study)
Ni Putu Mira Sumarta
Department of Oral & Maxillofacial Surgery, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia. Email: [email protected]
Coen Pramono D
Department of Oral & Maxillofacial Surgery, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia
Eryk Hendrianto
Stem cell Research and Development Center, Airlangga University, Surabaya, Indonesia
Helen Susilowati
Stem cell Research and Development Center, Airlangga University, Surabaya, Indonesia
Deya Karsari
Stem cell Research and Development Center, Airlangga University, Surabaya, Indonesia
Fedik A.Rantam
Stem cell Research and Development Center, Airlangga University, Surabaya, Indonesia Virology and Immunology, Microbiology Department, Faculty of Veterinarian Medicine, Airlangga University, Surabaya, Indonesia
Background: Human umbilical cord mesenchymal stem cell is a promising source of allogenous MSC with great chondrogenic differentiation capacity. Meanwhile, platelet rich fibrin (PRF) is a natural fibrin matrix, rich in growth factors, forming a smooth and flexible fibrin network, supporting cytokines and cell migration, thus can be used as a scaffold that facilitate the differentiation of MSC. However, the differential capability of MSC cultured in PRF was still poorly understood. Method: We studied in vitro differentiation potential of MSC cultured in PRF by evaluating several markers such as FGF 18, Sox 9, type II collagen, aggrecan in 3 different culture medium. Result: The result showed that there was positive expression of FGF 18, Sox 9, type II collagen, aggrecan in all medium of in vitro culture. Conclusion: MSC cultured from human umbilical cord had the capacity of chondrogenic differentiation and able to produce cartilage extracellular matrix in vitro which means that hUCMSC is a potential allogeneic MSC for cartilage regeneration.
