ORIGINAL ARTICLE

Sleeve gastrectomy and pancreas omentoplasty improved β cell insulin expression and interleukin-1β serum level in non-obese diabetes mellitus rat

Erlangga Dimas , Abdul Mughni, Chemy Wiryawan, Vito Mahendra, Ignatius Riwanto

Erlangga Dimas
Trainee of Digestive Surgery-Faculty of Medicine, Universitas Diponegoro-Dr. Kariadi General Hospital Semarang, Indonesia. Email: [email protected]

Abdul Mughni
Digestive Surgery Division, Faculty of Medicine, Universitas Diponegoro-Dr. Kariadi General Hospital Semarang, Indonesia

Chemy Wiryawan
Resident of General Surgery, Faculty of Medicine, Universitas Diponegoro-Dr. Kariadi General Hospital Semarang, Indonesia

Vito Mahendra
Trainee of Digestive Surgery-Faculty of Medicine, Universitas Diponegoro-Dr. Kariadi General Hospital Semarang, Indonesia

Ignatius Riwanto
Digestive Surgery Division, Faculty of Medicine, Universitas Diponegoro-Dr. Kariadi General Hospital Semarang, Indonesia
Online First: April 30, 2021 | Cite this Article
Dimas, E., Mughni, A., Wiryawan, C., Mahendra, V., Riwanto, I. 2021. Sleeve gastrectomy and pancreas omentoplasty improved β cell insulin expression and interleukin-1β serum level in non-obese diabetes mellitus rat. Bali Medical Journal 10(1): 460-466. DOI:10.15562/bmj.v10i1.2258


Background: Diabetes mellitus is one of the health problems that is growing rapidly today. In Asia, majority of the population are non-obese with Type 2 Diabetes Mellitus (T2DM). Two main defects of T2DM, insulin resistance and impaired insulin secretion due to reduced pancreatic β cells. Persistent metabolic stress due to hyperglycemic conditions, causes inflammation of the islets of Langerhans. Interleukin-1β (IL-1β) signaling is the most frequent pathway inducing β cell dysfunction. The bariatric Sleeve Gastrectomy (SG) procedure increases particular hormone which stimulates β cell growth and pancreatic insulin production. Aside from the role as a blood glucose regulator, insulin also plays a role in anti-inflammatory modulation. Omentoplasty can be used to wrap organ or tissue structures. The Omentum has unique biological functions, including promote tissue regeneration and anti-inflammatory effect. The study evaluates the effect of Sleeve Gastrectomy and pancreas Omentoplasty procedures on β cell insulin expression and Interleukin-1β (IL-1β) serum levels in non-obese diabetes mellitus subjects.

Methods: Experimental study with "post-test control group design" on 27 rats with Diabetes Mellitus was divided into 3 groups: K (control), P1 (Sleeve Gastrectomy), P2 (Sleeve Gastrectomy + Omentoplasty). 10 days after procedure, we evaluated the insulin expression of β cells using monoclonal antibody anti-insulin stain and IL-1β serum levels using rat IL-1β Enzyme-Linked Immunosorbent Assay (ELISA) kit. Statistical analysis with Kruskal Wallis and Mann Whitney test. Correlation test with Spearman.

Results: β cell insulin expression increased in P2 (p=0.020), compared to control. While the IL-1β serum levels reduced significantly in all groups (p<0.001) compared to control. Moderate negative correlation (r = -0.476) between β cell insulin expression and IL-1β serum levels.

Conclusion: Sleeve gastrectomy and omentoplasty increased β cell insulin expression and decreased IL-1 β serum levels in non-obese rats with diabetes mellitus.

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