ORIGINAL ARTICLE

The effect of ethyl acetate fraction of Moringa oleifera leaves on neutrophil and MDA levels in the improvement of liver dysfunction in male rats with sepsis model

Riswanto Riswanto , Tatar Sumandjar, Dhani Redhono, Robby Kurniawan, Arief Rahman

Riswanto Riswanto
Departement of Tropical infection, Dr Moewardi Regional State Hospital/ Faculty of Medicine - Sebelas Maret University, Surakarta, Indonesia. Email: [email protected]

Tatar Sumandjar
Departement of Tropical infection, Dr Moewardi Regional State Hospital/ Faculty of Medicine - Sebelas Maret University, Surakarta, Indonesia

Dhani Redhono
Departement of Tropical infection, Dr Moewardi Regional State Hospital/ Faculty of Medicine - Sebelas Maret University, Surakarta, Indonesia

Robby Kurniawan
Departement of Tropical infection, Dr Moewardi Regional State Hospital/ Faculty of Medicine - Sebelas Maret University, Surakarta, Indonesia

Arief Rahman
Departement of Tropical infection, Dr Moewardi Regional State Hospital/ Faculty of Medicine - Sebelas Maret University, Surakarta, Indonesia
Online First: December 01, 2020 | Cite this Article
Riswanto, R., Sumandjar, T., Redhono, D., Kurniawan, R., Rahman, A. 2020. The effect of ethyl acetate fraction of Moringa oleifera leaves on neutrophil and MDA levels in the improvement of liver dysfunction in male rats with sepsis model. Bali Medical Journal 9(3): 721-724. DOI:10.15562/bmj.v9i3.1850


Purpose: Sepsis is life-threatening organ dysfunction caused by the dysregulation of the host response due to infection. Sepsis is caused by an imbalance between pro-inflammatory and anti-inflammatory cytokines. Moringa oleifera (Mo) leaves naturally contains antiinflammation and antioxidant. We aim to investigate Mo leaves in sepsis.

Patients and methods: This is an experimental laboratory study with Post Test Only Control Group Design. 30 Winstar males rats were divided into four treatment groups; each consist of 6 rats. The negative group, the positive control group received an injection of LPS dose of 0.25 mg/kg BW, and the interventional group with Mo-EA dose of 10, 20, 40 mg/kg BW. The Annova and Kruskal-Wallis test was used in this study. The significant p-value<0.05. The measured outcome were neutrophil levels, malondialdehyde (MDA) levels, and Serum Glutamic Pyruvate Transaminase (SGPT) / Serum Glutamic Oxaloacetic Transaminase (SGOT).

Results: The neutrophil, MDA, SGOT, and SGPT levels were decreased significantly within the treated group. MO Fraction can decrease the serum of MDA, Neutrophil, and SGOT/SGPT levels in days 3 and 7 (p<0.05).

Conclusion: There was a decrease in the level of Neutrophil, MDA, SGOT, and SGPT improvement of Hepar organ dysfunction after administration of MO Extract, so it is useful to inhibit the progression of sepsis.

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