ORIGINAL ARTICLE

Correlation of neutrophils lymphocytes ratio with femur muscle damage due to acute limb ischemia in white Wistar rats

Yopie Afriandi Habibie , Avisenna Gatot Purnomo

Yopie Afriandi Habibie
Division of Thoracic Cardiac and Vascular Surgery, Department of Surgery, Faculty of Medicine, Universitas Syiah Kuala, The Dr. Zainoel Abidin General Hospital, Banda Aceh, Indonesia. Email: [email protected]

Avisenna Gatot Purnomo
Residency in Surgery Program, Faculty of Medicine, Universitas Syiah Kuala, The Dr. Zainoel Abidin General Hospital, Banda Aceh, Indonesia
Online First: February 02, 2020 | Cite this Article
Habibie, Y., Purnomo, A. 2020. Correlation of neutrophils lymphocytes ratio with femur muscle damage due to acute limb ischemia in white Wistar rats. Bali Medical Journal 9(1): 99-103. DOI:10.15562/bmj.v9i1.1698


Background: Acute limb ischemia (ALI) is a sudden decrease in limb perfusion that threatens limb viability and requires urgent evaluation and management. Assessment determines whether the limb is viable or irreversibly damaged. Neutrophil-lymphocyte ratio (NLR) is a useful marker for cardiovascular disease to delineate the prognosis. Endothelial dysfunctions related to atherosclerotic plaques are usually associated with states of neutrophilia together with lymphopenia processes.

Methods: This study was a post-test only control group performed on Wistar strain white rats. Thirty rats were divided into 5 groups, Control group (K), treatment group one (P1) examined NLR and femoral muscle tissue after 3 hours of ALI, P2 after 4 hours of ALI, P3 after 5 hours of ALI and P4 after 6 hours of ALI. Muscle tissue was histologically examined.

 Results: The duration of ALI increases the NLR value in Wistar strain white rats, the NLR value examined 6 hours after the rats experienced ALI increased significantly compared with the NLR values examined in the control group, the group examined 3 hours, 4 hours and 5 hours after the rats had ALI (p = 0.004). Necrotic muscle tissue very strongly correlated with the duration of ALI (r = 0.860, p<0.001). NLR value is strongly correlated with muscle tissue damage (r = 0.634, p<0.001).

 

Conclusion: The duration of ALI increases the NLR value in wistar strain white rats, NLR values increased significantly after 6 hours of ALI. Necrotic muscle tissue occurs after six hours of ALI. NLR value is strongly correlated with muscle tissue damage. Revascularization must be done within 6 hours to prevent permanent damage.

 

 

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