ORIGINAL ARTICLE

The ethyl acetate fraction of Moringa oleifera leaves effects on endothelial stress in rat sepsis model

Tatar Sumandjar , Bambang Purwanto, Brian Wasita, Dono Indarto, Risya Cilmiaty, Vitri Widyaningsih

Tatar Sumandjar
Department of Internal Medicine Dr. Moewardi General Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia. Email: [email protected]

Bambang Purwanto
Department of Internal Medicine Dr. Moewardi General Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Brian Wasita
Department of Anatomical Pathology Dr. Moewardi General Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Dono Indarto
Department of Physiology and Biomedical Laboratory Dr. Moewardi General Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Risya Cilmiaty
Department of Dental and Oral Medicine Dr. Moewardi General Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Vitri Widyaningsih
Department of Public Health Dr. Moewardi General Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Online First: December 26, 2019 | Cite this Article
Sumandjar, T., Purwanto, B., Wasita, B., Indarto, D., Cilmiaty, R., Widyaningsih, V. 2019. The ethyl acetate fraction of Moringa oleifera leaves effects on endothelial stress in rat sepsis model. Bali Medical Journal 8(3): 940-943. DOI:10.15562/bmj.v8i3.1679


Background: Sepsis is a life-threatening organ dysfunction caused by the dysregulation of the host response to infection, facilitated by inflammation and endothelial stress. Moringa oleifera leaves fractioned with ethyl acetate (MO-EA fraction) is proposed to have the ability to suppress inflammation and oxidative stress through the control of NF-kB. This study aims to investigate the effect of the MO-EA fraction in a rat sepsis model.

Material and Methods: The study is a laboratory experimental study. The research was conducted on a total of 30 rats, equally divided into five groups. One group did not receive lipopolysaccharide (LPS) induction as negative control group, but the others received LPS induction and received variable doses of MO-EA fraction (0, 10, 20, and 40 mg/kg of bodyweight). The measured outcome were serum concentration of of heparanase, CRP, malondialdehyde (MDA), and immunohistologic expression of e-selectin, NF-kB in cells, and histopathological necrosis in the aorta and kidney.

Results: MO-EA fraction significantly decrease the serum levels of HPA, MDA on day 3 and 7, and lower the CRP serum level on day 7 (p<0.05). Other variables of interest did not show significant differences.

Conclusion: The administration of MO-EA fraction of any dose significantly lower the serum level of HPA and MDA in mice sepsis models on day 3 and 7, and lower CRP on day 3 but not on day 7. However, examnationa of NF-kB and e-selectin expressions, and necrosis in kidney proximal tubule and aortic endothelial cells did not show the benefit of MO-EA fraction in preventing cell damage.

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