ORIGINAL ARTICLE

Differences of locomotor activities in the dementia rat model induced by oral d-galactose and intraperitoneal injection

Ketut Widyastuti, Anak Agung Ayu Putri Laksmidewi, I Made Oka Adnyana , Dewa Putu Gde Purwa Samatra

Ketut Widyastuti
Department of Neurology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia

Anak Agung Ayu Putri Laksmidewi
Department of Neurology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia

I Made Oka Adnyana
Department of Neurology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia. Email: [email protected]

Dewa Putu Gde Purwa Samatra
Department of Neurology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
Online First: April 01, 2020 | Cite this Article
Widyastuti, K., Laksmidewi, A., Adnyana, I., Samatra, D. 2020. Differences of locomotor activities in the dementia rat model induced by oral d-galactose and intraperitoneal injection. Bali Medical Journal 9(1): 216-218. DOI:10.15562/bmj.v9i1.1651


Background: The aging process can increase the incidence of neurodegenerative diseases such as dementia. Dementia is characterized by a gradual loss of cognitive performance for locomotor activity in exploring new environments. Research on dementia in mice was carried out by administering d-galactose to induce mitochondrial dysfunction, oxidative stress, inflammation and apoptosis of neurons.

Objective: The purpose of this study was to determine differences in locomotor activity in rats induced by oral d-galactose and intraperitoneal injection.

Methods: An experimental study with a posttest control group design. The subjects were male Wistar rats aged 12-14 weeks with weighing 200-300gram obtained from the Animal Laboratory of the Pharmacology Unit of the Faculty of Medicine, Udayana University. The study was conducted for 8 weeks, from June to August 2019. A total of 20 rats induced d-galactose dose of 100 mg/kg/day orally and intraperitoneal injection. The use of animals was carried out after obtaining ethical clearance from the ethical committee of the Faculty of Medicine, Udayana University. Y maze test is conducted for 5 minutes at the end of week 8.

Results: There was a decrease in locomotor activity in both groups. The average locomotor activity in the oral group was 6.5+0.268, and the injection group was 5.5+0.341. Bivariate analysis with unpaired t-test showed significant differences in locomotor activity between groups given oral d-galactose compared to the intraperitoneal injection group with p=0.034 (<0.05).

Conclusion: Alzheimer's dementia induced d-galactose mice to have a different locomotor activity after administration by oral or intraperitoneal injection of d-galactose. This study supports the results of previous studies that the administration of d-galactose can cause impaired locomotor activity.

References

Parameshwaran K, Irwin MH, Steliou K, Pinkert CA. D-Galactose Effectiveness in Modeling Aging and Therapeutic Antioxidant Treatment in Mice. Rejuvenation Research Vol. 13. No. 6. 2010;729-735

Zhu Q, Ren X, Zhang C. Effects of d-galactose on the structure of nerve fibers in cerebellar white matter. Pakistan J. Zool. 2015. 47(1):187–92.

Woodruff-Pak DS, Foy MR, Akopian GG, Lee KH, Zach J, Nguyen KPT, et al. Differential effects and rates of normal aging in cerebellum and hippocampus. P.N.A.S. 2010. 107(4):1624-9.

Rodrigues AF, Biasibetti H, Zanotto BS, Sanches EF, Schmitz, Nunes VT, et al. D-Galactose causes motor coordination impairment, and histological and biochemical changes in the cerebellum of rats. Mol. Neurobiol. 2016. p1–11.

Sharma S, Rakoczy S, Brown-Borg H. Assessment of spatial memory in mice. Life Sciences. 2010. 87, 521-536

Austin AR. Effects of D-Galactose treatment and moderate exercise on spatial memory in rats. Western Michigan University. 2012.

Chogtu B, ArivazhahanA, Kunder SK, Tilak A, Sori R, Tripathy A. Evaluation of Acute and Chronic Effects of D-Galactose on Memory and Learning in Wistar Rats. Clinical Psychopharmacology and Neuroscience. 2018;16(2):153-160.

Cardoso A, Sara Magano S, Marrana F, Jose, Andrade P. D-Galactose High-Dose Administration Failed to Induce Accelerated Aging Changes in Neurogenesis, Anxiety, and Spatial Memory on Young Male Wistar Rats. Rejuvenation Research. 2015;18(6):497-507.

Budni J, Pacheco R, Silva S, Garcez ML, Mina F, Bellettini-Santos T, et al. Oral administration of d-galactose induces cognitive impairments and oxidative damage in rats. Behav Brain Res 2016; 302:35-43.

Song X, Bao M, Li D, Li YM. Advanced glycation in d-galactose induced mouse aging model. Mech. Aging Dev. 1999;108(3):239–51.

Zhang C, Zhu Q, Hua T. Aging of cerebellar Purkinje cells. Cell Tissue Res. 2010;341(3):341–47.


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