ORIGINAL ARTICLE

SPILANTHES ACMELLA AND PHYSICAL EXERCISE INCREASED TESTOSTERONE LEVELS AND OSTEOBLAST CELLS IN GLUCOCORTICOID-INDUCED OSTEOPOROSIS MALE MICE

Hening Laswati , Imam Subadi, Retno Widyowati, Mangestuti Agil, Jahya Alex Pangkahila

Hening Laswati
Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Airlangga University, Surabaya-Indonesia. Email: [email protected]

Imam Subadi
Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Airlangga University, Surabaya-Indonesia

Retno Widyowati
Department pf Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya-Indonesia

Mangestuti Agil
Department pf Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya-Indonesia

Jahya Alex Pangkahila
Department of Andrology and Sexology, Faculty of Medicine, Udayana University, Bali-Indonesia
Online First: August 22, 2015 | Cite this Article
Laswati, H., Subadi, I., Widyowati, R., Agil, M., Pangkahila, J. 2015. SPILANTHES ACMELLA AND PHYSICAL EXERCISE INCREASED TESTOSTERONE LEVELS AND OSTEOBLAST CELLS IN GLUCOCORTICOID-INDUCED OSTEOPOROSIS MALE MICE. Bali Medical Journal 4(2): 76-81. DOI:10.15562/bmj.v4i2.124


Background: Glucocorticoid-induced osteoporosis is leading cause of secondary osteoporosis by decreasing formation activity and increasing resorption activity. Spilanthes acmella, is one of Indonesia medicinal plants that contain of polyphenol and flavonoids. Previously in vitro study showed that buthanol and water fraction from this plant have increased alkaline phosphatase that known as marker of bone formation. The objective of this study to analyze the effect of Spilanthes acmella  and physical exercise in increasing testosterone and  osteoblast cells of femoral’s trabecular glucocorticoid-induced osteoporosis male mice. Method: This study using a posttest control group design, 36 male healthy mice (5 months old)  were randomizely devided into 6 groups, there are : 1.Healthy control group (without induction dexamethaxone), 2.Osteoporosis groups (induction with dexamethaxone without treatment), 3.Positive control receive suspension alendronat, 4.70% Ethanol extract of Spilanthes acmella group, 5.Combination group of 70% extract ethanol of Spilanthes acmella and exercise, and 6.Exercise group  (walking using mice treadmill 10m/minute, 5-12 minutes 3 times a week). All of the intervention were given for 4 weeks. The serum levels of testosterone were determined using  immunoserology (ELISA) and osteoblast cells were determined histomorphometry by light microscopy.  All statistical test were carried out using SPSS 23 and statistical significance was  set at p<0.05 for all analysis. The testosterone levels  between group were compared using Mann-Whitney test and osteoblast cells between group were compared with multiple comparison. Results: It showed that the alendronate group, combination group and the exercise group increasing testosterone level (p<0.05) from that osteoporotic group. There were also increasing osteoblast cells (p<0.05) in the alendronate group and combination group. There was no correlation between testosterone level and osteoblast  cells (p>0.05). Conclusion: It proved that 70% ethanol extract of Spilanthes acmella have an additive effect to weight bearing exercise in glucocorticoid-induced osteoporosis male mice. 

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