Laser acupuncture on ST36 promotes serum protein concentration in adolescent rats

Selfi Handayani , Ari Handono Ramelan, Kristanto Yuli Yarsa, Dono Indarto, Bambang Purwanto, Koosnadi Saputro, Didik G Tamtomo

Selfi Handayani
Doctorate Program in Medical Sciences, Postgraduate Universitas Sebelas Maret, Surakarta, Indonesia. Email: [email protected]

Ari Handono Ramelan
Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Surakarta, Indonesia.

Kristanto Yuli Yarsa
Department of Surgery, Oncology Division Medical Faculty of Sebelas Maret University Solo-Indonesia Department of Surgery, Division of Oncologic Surgery, Faculty of Medicine Udayana University Bali-Indonesia.

Dono Indarto
Biomedical Laboratory, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Bambang Purwanto
Department of Internal Medicine Dr Moewardi Hospital/ Facuty of Medicine, Universitas Sebelas Maret

Koosnadi Saputro
Acupuncture Research Laboratory for Health Services, National Institute of Health of Republic Indonesia/Academy Acupuncture of Surabaya. Indonesia

Didik G Tamtomo
Department of Anatomy, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Online First: October 03, 2018 | Cite this Article
Handayani, S., Ramelan, A., Yarsa, K., Indarto, D., Purwanto, B., Saputro, K., Tamtomo, D. 2018. Laser acupuncture on ST36 promotes serum protein concentration in adolescent rats. Bali Medical Journal 7(3). DOI:10.15562/bmj.v7i3.1170

Background: In the last decade, laser technology has been used in medicine for diagnostic and therapeutic purposes. The low-level laser has been applied to acupoints (laser acupuncture/LA) which had potential to stimulate the expression of enzyme and hormone in various biological processes. Since the enzyme and majority of hormone is a protein or its derivatives, this study aimed to measure serum protein concentration in adolescent rats treated with laserpuncture.

Methods: Forty male three weeks old Wistar rats weighed ≥ 40 g were randomly divided into either group A or B. Each group was evenly divided into four subgroups which were a negative control and treated with LA on GV20, ST 36 or combination of GV 20+ST 36 respectively. These acupoints were then stimulated by continuous wave cold red laser (635-680nm/5mW) generated by KX Laser GX-2000B. LA was performed at 60 seconds/day for ten days (group A) and 15 days (group B). Venous blood was collected from sinus orbitalis, and Serum protein concentration was measured using the Bradford assay. The data were analyzed using ANOVA test and p-value < 0.05 was considered as significant. Results: A higher protein concentration was observed in ST36A group (76.326±15.084 mg/ml) and GV20+ST36A group (75.044±15.346 mg/ml) compared with control group A (67.338±12,476 mg/ml).

Meanwhile,GV20A group had lower protein level than control group at 57.838±11.042 mg/ml. By contrast, all treated rats in group B had higher protein concentrations (74.158±12.754; 79.373±7.265 and 77.854±2.552 mg/ml respectively) compared to control group B (72.292±7.987 mg/ml). However, the difference in protein concentrations in both groups did not statistically significantly (p=0.161 and p=0.541 respectively). Conclusion: LA on the ST 36 acupoint has the greatest effect on increasing protein concentration in male adolescent Wistar rats although the difference was not statistically significant.


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