ORIGINAL ARTICLE

Optimising the effect of activated carbon on the microrheological properties of erythrocyte in rats with experimentally developed obesity

Evgeniy Vladimirovich Kulikov, Svetlana Yurevna Zavalishina , Yuri Anatolyevich Vatnikov, Alexander Anatolyevich Strizhakov, Stanislav Gennadievich Drukovsky, Dmitry Anatolyevich Lozovoy, Yuliya Yurevna Voronina, Irina Anatolyevna Popova, Irina Viktorovna Bondareva, Natalia Borisovna Sambros, Abdelaziz Norezzine, Tatyana Ivanovna Glagoleva, Svetlana Alexandrovna Shemyakova

Evgeniy Vladimirovich Kulikov
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Svetlana Yurevna Zavalishina
Russian State Social University, Moscow, Russia. Email: [email protected]

Yuri Anatolyevich Vatnikov
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Alexander Anatolyevich Strizhakov
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Stanislav Gennadievich Drukovsky
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Dmitry Anatolyevich Lozovoy
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Yuliya Yurevna Voronina
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Irina Anatolyevna Popova
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Irina Viktorovna Bondareva
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Natalia Borisovna Sambros
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Abdelaziz Norezzine
Peoples Friendship University of Russia (RUDN University) 6 Miklukho-Maklaya Street, Moscow, Russia

Tatyana Ivanovna Glagoleva
All-Russian Research Institute of Physiology, Biochemistry and Nutrition of Animals, Institute of Village, Borovsk, Russia

Svetlana Alexandrovna Shemyakova
Moscow state Academy of veterinary medicine and biotechnology - MVA by K.I.Scryabin, Moscow, Russia
Online First: December 01, 2019 | Cite this Article
Kulikov, E., Zavalishina, S., Vatnikov, Y., Strizhakov, A., Drukovsky, S., Lozovoy, D., Voronina, Y., Popova, I., Bondareva, I., Sambros, N., Norezzine, A., Glagoleva, T., Shemyakova, S. 2019. Optimising the effect of activated carbon on the microrheological properties of erythrocyte in rats with experimentally developed obesity. Bali Medical Journal 8(3). DOI:10.15562/bmj.v8i3.1112


Background: Remains not fully clear the impact of intestinal chelators on microrheological violation of red blood cells during the early stages of obesity. It dictates the necessity of experimental investigations' fulfilment on laboratory animals with just developed obesity in them.

Method: Ninety-four of healthy male-rats of Vistar line at the age of 2.5-3 months took into investigation. Twenty-nine animals of them had experienced no impacts and composed the control group. In 65 rats after their putting into small cages (one specimen - in a cage) during 30 days there was developed OB as the result of giving them of high-caloric diet from combined feed (47%), sweet condensed milk (44%), vegetable oil (8%) and vegetable starch (1%). Of the 35 rats received a day for 60 days of activated carbon 25 mg/kg into a stomach. There used biochemical, haematological and statistical methods of investigation.

Results: At obesity development in rats, there found a steady decrease of erythrocytes-discocytes quantity in blood. It accompanied by increase of reversibly and irreversibly changed erythrocytes' quantity in examined animals' blood. At obesity development in rats there was found quick rise of erythrocytes' sum in aggregate and these aggregates' quantity at lowering of free erythrocytes' number. During experimental obesity modelling we noticed very early in rats' blood decrease of erythrocytes-discocytes' quantity, level rise of their reversibly and irreversibly varieties with strengthening of their aggregative ability. It takes place in the background of weakening of erythrocytes' antioxidant protection and activation of lipids' peroxidation in them.

Conclusion: Application of activated carbons capable of rats with experimental obesity resolve arisen they breach microrheological properties of erythrocytes. It is clear that in obesity treatment regimens must be intestinal chelators, capable of positively affect many broken with this pathology haematological indices.

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