ORIGINAL ARTICLE

A preliminary study of the effect of PPAR- γ agonist from Myristica fragrans houtt seed extract on the biogenesis of rat infant’s brain mitochondria and D1 dopamine receptor

Fifi Veronica, Leo Lubis , Arifin S, Lulu L Fitri, A Rizal, Ambrosius P, Hanna Gunawan, Ronny Lesmana, Keri Lestari Dandan, Unang Supratman

Fifi Veronica
Department of Anatomy, Physiology and Cell Biology, University of Padjajaran, Bandung

Leo Lubis
Department of Anatomy, Physiology and Cell Biology, University of Padjajaran, Bandung. Email: [email protected]

Arifin S
Department of Anatomy, Physiology and Cell Biology, University of Padjajaran, Bandung

Lulu L Fitri
School of Life Sciences and Technology - ITB Bandung

A Rizal
Department of Neurology RSHS-Bandung

Ambrosius P
Department of Anatomy, Physiology and Cell Biology, University of Padjajaran, Bandung

Hanna Gunawan
Department of Anatomy, Physiology and Cell Biology, University of Padjajaran, Bandung

Ronny Lesmana
Department of Anatomy, Physiology and Cell Biology, University of Padjajaran, Bandung

Keri Lestari Dandan
Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjajaran, Bandung

Unang Supratman
Biology Activity Division,Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia
Online First: October 03, 2018 | Cite this Article
Veronica, F., Lubis, L., S, A., Fitri, L., Rizal, A., P, A., Gunawan, H., Lesmana, R., Dandan, K., Supratman, U. 2018. A preliminary study of the effect of PPAR- γ agonist from Myristica fragrans houtt seed extract on the biogenesis of rat infant’s brain mitochondria and D1 dopamine receptor. Bali Medical Journal 7(3). DOI:10.15562/bmj.v7i3.1027


Background: Myristica fragrans (nutmeg) seed extract (NuSE) has been reported as a ligand for Peroxisome Proliferator-Activated Receptor (PPAR)-γ due to hypoglycemic and antidiabetic effects. The recent study also finds the role of PGC-1α as the regulator of mitochondrial biogenesis and the co-activator of PPAR-γ as well. Dopamine 1 receptor (D1DR) is generally distributed in the brain with a various function, such as cognitive function. As a preliminary study, we explore a possibility the current knowledge of the mechanism of NuSE enhance dopamine 1 receptor via neuronal mitochondrial biogenesis on infant rat brain tissue. Aim: This study was aimed to investigate whether PPAR- γ agonist from NuSE can induce expression brain PGC-1α and D1DR (D1A and D1B). Methods: Seven male rats (4 weeks) as a control and another seven male rats (4 weeks) was giving 8.1 mg NuSE /day by gavage within 12 weeks. End of the day, the rats were sacrificed, and the forebrain was extraction. Expression mRNA PGC 1-α and DIDR  were analyzed. Results: The result showed significantly higher relatives expression of mRNA PGC-1α and mRNA D1DR in NuSE group (p<0.05). Conclusion: The findings provide preliminary support that PPAR-γ agonist from NuSE may offer a novel for the study brain mitochondrial biogenesis related mechanism of signaling dopamine receptor.

 

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